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Doctoral Fellowships


Development of cell-seeded TE valves using decellularised valvular scaffolds (ESR6)


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The aim of this Fellowship is to explore the feasibility of developing a living valve replacement using decellularised human or porcine semilunar valves, reseeded with human cells and physically conditioned in a bioreactor. Aortic and pulmonary valves will be decellularised according to previously established protocols, and will be functionalised with a cohort of different bioactive peptides to enhance cell colonisation. The functionalised scaffolds will be assessed against their untreated counterparts in terms of structural/biomechanical alterations, surface morphology, function and durability in collaboration with other ESR projects within the TECAS-ITN. The functionalised scaffolds will be seeded statically with either human stromal cells, or bone marrow stem cells to optimise cell/scaffold-type combination and seeding conditions. Optimally-seeded scaffolds will be conditioned in a pulsatile bioreactor under different conditions to optimise valve regeneration. Statically-seeded and conditioned valves will be assessed in terms of cell phenotype, structural/biomechanical integrity and neo-matrix synthesis to assess the effect of dynamic conditioning on valve regeneration.

 

 

 

Research Field: Cardiovascular tissue engineering and regenerative medicine

   

Collaborators: RWTH Aachen University and Hospital, University of Padua, University of Patras, Corlife, Eindhoven University of Technology

   

Supervision: Dr. Sotirios Korossis (PI), Dr. Sergei Cebotari (co-I), Dr. Andres Hilfiker (co-I), Prof. Stefan Jockenhövel (co-I), Prof. Gino Gerosa (co-I), Prof. Demosthenes Mavrilas (co-I), Prof. Frank Baaijens (co-I)

 


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EVENTS

TECAS-ITN End-of-Network Conference

 Padua, November 28-30, 2016

The TECAS-ITN European Doctoral Academy in Regenerative Engineering, is organising the End-of-Network Conference in Padua, Italy.


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