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Doctoral Fellowships

Experimental investigation of α-actin remodelling (ESR2)



The aim of this Fellowship is to develop an experimental system, which will be able to induce uniaxial or biaxial dynamic loading on cell-seeded scaffolds, with a view to measuring cell-induced traction forces. The setup will be designed to enable visualisation and inspection of cellular properties, such as α-actin and viability, and collagen orientation, using staining in a 3D setting. The work will focus on the development of collagen- and fibrin-based micro-tissue model systems, employing micro-fabrication technology and heart valve interstitial cells, which will be integrated in a cyclic stretching device under aseptic conditions. Subsequently, α-actin and collagen fibre orientation and distribution will be visualised and recorded under cyclic strain using confocal microscopy, and the cellular-level traction forces generated will be quantified using cantilever deflection measurements. The α-actin and collagen fibre architecture will be compared against histological sections of full-scale bioreactor-conditioned tissues and, together with the measured cellular-level forces, in the validation of computational models developed in other projects within the TECAS-ITN.




Research Field: Cardiovascular tissue engineering and regenerative medicine


Collaborators: RWTH Aachen University and Hospital, Hannover Medical School, University of Padua.

Supervision: Prof. Frank Baaijens (PI), Prof. Carlijn Bouten (co-I), Dr. Sotirios Korossis (co-I), Prof. Stefan Jockenhövel (co-I), Prof. Gino Gerosa (co-I) 


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TECAS-ITN End-of-Network Conference

 Padua, November 28-30, 2016

The TECAS-ITN European Doctoral Academy in Regenerative Engineering, is organising the End-of-Network Conference in Padua, Italy.

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